KEY: ENSP* - Homo sapiens (Human) ENSBTA* - Bos taurus (Cow) ENSTTR* - Tursiops truncatus (Dolphin) bmy_* - Balaena mysticetus (Bowhead whale) BACU* - Balaenoptera acutorostrata (Minke whale) Copy from below the line for a valid .msf file. ---------------------------------------------------------------------------------- NoName MSF: 5 Type: P Thu May 15 18:22:59 2014 Check: 00 .. Name: ENSP00000295736/1-1214 Len: 1798 Check: 210 Weight: 1.00 Name: ENSBTAP00000053881/1-1218 Len: 1798 Check: 1847 Weight: 1.00 Name: bmy_08829T0/1-1141 Len: 1798 Check: 8897 Weight: 1.00 Name: BACU014668/1-1379 Len: 1798 Check: 9918 Weight: 1.00 Name: ENSTTRP00000010232/1-1198 Len: 1798 Check: 7613 Weight: 1.00 // 1 50 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 SESIQFLLCL CCVVNFPDFK NFSFSFFYKL RMKAFSLKTQ TLTIKLYIHL BACU014668/1-1379 ---------- ---------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 51 100 ENSP00000295736/1-1214 ------MERF RLEKKLP--- --GPDEEAVV DLGKTSSTVN TKFEKEELES ENSBTAP00000053881/1-1218 ------MEAD GTGEQMRPLL TRGPDEEAVV DLGKTSSTVN TKFEKEELES bmy_08829T0/1-1141 ALSHLLQASQ RVSKESK--- --GPDEEAVV DLGKTSSTVN TKFEKEELE- BACU014668/1-1379 ------MERF RLEKKLP--- --GPDEEAVV DLGKTSSTVN TKFEKEELES ENSTTRP00000010232/1-1198 ---------- ---------- --GPDEEAVV DLGKTSSTVN TKFEKEELES 101 150 ENSP00000295736/1-1214 HRAVYIGVHV PFSKESRRRH RHRGHKHHHR RRKDKESDKE DGRESPSYDT ENSBTAP00000053881/1-1218 HRAVYIGVHV PFSKESRRRH RHRGHKHHHR RRKDKDSDRE DGRESPSYDT bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- --------NT BACU014668/1-1379 HRAVYIGVHV PFSKESRRRH RHRGHKHHHR RRKDKDSDRE DGRESPSYDT ENSTTRP00000010232/1-1198 HRAVYIGVHV PFSKESRRRH RHRGHKHHHR RRKDKDSDRE DGRESPSYDT 151 200 ENSP00000295736/1-1214 PSQRVQFILG TEDDDEEHIP HDLFTEMDEL CYRDGEEYEW KETARWLKFE ENSBTAP00000053881/1-1218 PSQRVQFILG TEDDDEEHIP HDLFTEMDEL CYRDGEEYEW KETARWLKFE bmy_08829T0/1-1141 PSQRVQFILG TEDDDEEHIP HDLFTEMDEL CYRDGEEYEW KETARWLKFE BACU014668/1-1379 PSQRVQFILG TEDDDEEHIP HDLFTEMDEL CYRDGEEYEW KETARWLKFE ENSTTRP00000010232/1-1198 PSQRVQFILG TEDDDEEHIP HDLFTEMDEL CYRDGEEYEW KE---WLKFE 201 250 ENSP00000295736/1-1214 EDVEDGGDRW SKPYVATLSL HSLFELRSCI LNGTVMLDMR ASTLDEIADM ENSBTAP00000053881/1-1218 EDVEDGGDRW SKPYVATLSL HSLFELRSCI LNGTVMLDMR ASTLDEIADM bmy_08829T0/1-1141 EDVEDGGDRW SKPYVATLSL HSLFELRSCI LNGTVMLDMR ASTLDEIADM BACU014668/1-1379 EDVEDGGDRW SKPYVATLSL HSLFELRSCI LNGTVMLDMR ASTLDEIADM ENSTTRP00000010232/1-1198 EDVEDGGDRW SKPYVATLSL HSLFELRSCI LNGTVMLDMR AGTLDEIADM 251 300 ENSP00000295736/1-1214 VLDNMIASGQ LDESIRENVR EALLKRHHHQ NEKRFTSRIP LVRSFADIGK ENSBTAP00000053881/1-1218 VLDNMIASGQ LDESIRENVR EALLKRHHHQ NEKRFTSRIP LVRSFADIGK bmy_08829T0/1-1141 VLDNMIASGQ LDESIRENVR EALLKRHHHQ NEKRFTSRIP LVRSFADIGK BACU014668/1-1379 VLDNMIASGQ LDESIRENVR EALLKRHHHQ NEKRFTSRIP LVRSFADIGK ENSTTRP00000010232/1-1198 VLDNMIASGQ LDESIRENVR EALLKRHHHQ NEKRFTSRIP LVRSFADIGK 301 350 ENSP00000295736/1-1214 KHSDPHLLER NGEGLSASRH SLRTGLSASN LSLRGESPLS LLLGHLLPSS ENSBTAP00000053881/1-1218 KHSDPHLLER NGEGLSASRH SLRTGLSASN LSLRGESPLS LLVSHLLPSS bmy_08829T0/1-1141 KHSDPHLLER N--------- ---------- ---------- ---------- BACU014668/1-1379 KHSDPHLLER NGEGLSASRH SLRTGLSASN LSLRGESPIS LLLSHLLPSS ENSTTRP00000010232/1-1198 KHSDPHLLER NGEGLSASRH SLRTGLSASN LSLRGESPLS LLLSHLLPSS 351 400 ENSP00000295736/1-1214 RAGTPAGSRC TTPVPTPQNS PPSSPSISRL TSRSSQESQR QAPELLVSPA ENSBTAP00000053881/1-1218 RAGTPAGSRC TSPAPTPQSS PPSSPSLSRL HARASRQRQP QAPELLVSPA bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ---------- BACU014668/1-1379 RAGTPAGSKC TTPVPTPQNS PPSSPRLSRL NSRSSQQRQL QAPELLVSPA ENSTTRP00000010232/1-1198 RAGTPAGSKC TTPVPTPQNS PPSSPRLSRL NSRSSQQRQL QAPELLVSPA 401 450 ENSP00000295736/1-1214 SDDIPTVVIH PPEEDLEAAL KGEEQKNEEN VDLTP----- ----GILASP ENSBTAP00000053881/1-1218 SAGVPTVVIH PPEEDLE-AL QGQEQKTEEN VDVTA----- ----GILASP bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ----GILASP BACU014668/1-1379 SDDIPTVVIH PPEEDLE-AL QGQEQKNEEN VDITSGNYSK SSMCGILASP ENSTTRP00000010232/1-1198 SDDIPTVVIH PPEEDLE-AL RGQEQKNEEN VDITS----- ----GILASP 451 500 ENSP00000295736/1-1214 QSAPGNLDNS KSGEIKGNGS GGSRENSTVD FSKVDMNFMR KIPTGAEASN ENSBTAP00000053881/1-1218 QSAPGNLDNS KSGEMKGNGS GGSRENSTVD FSKVDMNFMR KIPSGAEASN bmy_08829T0/1-1141 QSAPGNLDNS KSGEMKGNGS GGSRENSTVD FSKVDMNFMR KIPSGAEASN BACU014668/1-1379 QSAPGNLDSS KSGEMKGNGS GGSRENSTVD FSKVDMNFMR KIPSGAEASN ENSTTRP00000010232/1-1198 QSAPGNLDNS KSGEIKGNGS GESRENSTVD FSKVDMNFMR KIPSGAEASN 501 550 ENSP00000295736/1-1214 VLVGEVDFLE RPIIAFVRLA PAVLLTGLTE VPVPTRFLFL LLGPAGKAPQ ENSBTAP00000053881/1-1218 VLVGEVDFLE RPIIAFVRLA PAVLLSGLTE VPVPTRFLFL LLGPAGKAPQ bmy_08829T0/1-1141 VLVGEVDFLE RPIIAFVRLA PAVLLSGLTE VPVPTRFLFL LLGPAGKAPQ BACU014668/1-1379 VLVGEVDFLE RPIIAFVRLA PAVLLSGLTE VPVPTRFLFL LLGPAGKAPQ ENSTTRP00000010232/1-1198 VLVGEVDFLE RPIIAFVRLA PAVLLSGLTE VPVPTRFLFL LLGPAGKAPQ 551 600 ENSP00000295736/1-1214 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD ENSBTAP00000053881/1-1218 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD bmy_08829T0/1-1141 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD BACU014668/1-1379 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD ENSTTRP00000010232/1-1198 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD 601 650 ENSP00000295736/1-1214 PSIRIEPPKS VPSQEKRKIP VFHNGSTPTL GETPKEAAHH AGPELQRTGR ENSBTAP00000053881/1-1218 PSIRIEPPKS VPSQEKRKIP VFPNGSAPSS GDAPKEAAHH AGPELQRTGR bmy_08829T0/1-1141 PSIRIEPPKS VPSQEKRKIP VFPNGSAPSS GETPKEAPHH AGPELQRTGR BACU014668/1-1379 PSIRIEPPKS VPSQ------ ---------- ---------- ---------- ENSTTRP00000010232/1-1198 PSIRIEPPKS VPSQEKRKIP VFPNGSAPSS GETPKEAPHH AGPELQRTGR 651 700 ENSP00000295736/1-1214 LFGGLILDIK RKAPFFLSDF KDALSLQCLA SILFLYCACM SPVITFGGLL ENSBTAP00000053881/1-1218 LFGGLILDIK RKAPFFLSDF KDALSLQCLA SILFLYCACM SPVITFGGLL bmy_08829T0/1-1141 LFGGLILDIK RKAPFFLSDF KDALSLQCLA SILFLYCACM SPVITFGGLL BACU014668/1-1379 ---------- ---------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 LFGGLILDIK RKAPFFLSDF KDALSLQCLA SILFLYCACM SPVITFGGLL 701 750 ENSP00000295736/1-1214 GEATEGRISA IESLFGASLT GIAYSLFAGQ PLTILGSTGP VLVFEKILYK ENSBTAP00000053881/1-1218 GEATEGRISA IESLFGASLT GIAYSLFAGQ PLTILGSTGP VLVFEKILFK bmy_08829T0/1-1141 GEATEGRISA IESLFGASLT GIAYSLFAGQ PLTILGSTGP VLVFEKILFK BACU014668/1-1379 ---------- ---------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 GEATEGRISA IESPFGASLT GIAYSLFAGQ PLTILRSTGP V-VYETILLK 751 800 ENSP00000295736/1-1214 FCRDYQLSYL SLRTSIGLWT SFLCIVLVAT DASSLVCYIT RFTEEAFAAL ENSBTAP00000053881/1-1218 FCRDYHLSYL SLRTSIGLWT SFLCIVLVAT DASSLVCYIT RFTEEAFAAL bmy_08829T0/1-1141 FCRDYHLSYL SLRTSIGLWT SFLCIVLVAT DASSLVCYIT RFTEEAFAAL BACU014668/1-1379 ---------- ---------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 FCRDYHLSYL SLRTSIGLWT SFLCIVLVAT DASSLVCYIT RFTEEAFAAL 801 850 ENSP00000295736/1-1214 ICIIFIYEAL EKLFDLGETY AFNMHNNLDK LTSYSCVCTE PPNPSNETLA ENSBTAP00000053881/1-1218 ICIIFIYEAL EKLFHLGETY AFNMHNNLEE LTSYSCVCVE PSNPGNETLK bmy_08829T0/1-1141 ICIIFIYEAL EKLFHLGEIY AFNMHNNLDE LTSYLCVCVE PSNPGNETLK BACU014668/1-1379 ---------- ---------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 ICIIFIYEAL EKLFHLGEIY AFNMHNNLDE LTSYSCVCVE PSNPGNETLK 851 900 ENSP00000295736/1-1214 QWKKDNITAH NISWRNLTVS ECKKLRGVFL GSACGHHGPY IPDVLFWCVI ENSBTAP00000053881/1-1218 MWEKNNVTAR DIPWGNLTVS ECKVYHGTFV GSACSLHGPY IPDVLFWSVI bmy_08829T0/1-1141 VWEERNVTAH NIPWGNLTVS ECKIFHGTFV GSACSLHGPY IPDVLFWCVI BACU014668/1-1379 ---------- ---------- ECKIFHGTFV GSACSLHGPY IPDVLFWCVI ENSTTRP00000010232/1-1198 VWEERNVTAH NIPWGNLTVS ECKIFHGTFV GSACSLHGPY IPDVLFWCVI 901 950 ENSP00000295736/1-1214 LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVTIDYLVGV ENSBTAP00000053881/1-1218 LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVVIDYLVGV bmy_08829T0/1-1141 LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVAIDYLVGV BACU014668/1-1379 LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVAIDYLVGV ENSTTRP00000010232/1-1198 LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVAIDYLVGV 951 1000 ENSP00000295736/1-1214 PSPKLHVPEK FEPTHPERGW IISPLGDNPW WTLLIAAIPA LLCTILIFMD ENSBTAP00000053881/1-1218 PSPKLHVPEK FEPTDKSRGW IISPLGENPW WTLLIAAIPA LLCTILIFMD bmy_08829T0/1-1141 PSPKLHVPEK FEPTDTNRGW IISPLGENPW WTLLIAAIPA LLCTILIFMD BACU014668/1-1379 PSPKLHVPEK FE-------- ---------- ---------- ---------- ENSTTRP00000010232/1-1198 PSPKLHVPEK FEPTDTNRGW IISPLGENPW WTLLIAAIPA LLCTILIFMD 1001 1050 ENSP00000295736/1-1214 QQITAVIINR KEHKLKKGAG YHLDLLMVGV MLGVCSVMGL PWFVAATVLS ENSBTAP00000053881/1-1218 QQITAVIINR KEHKLKKGAG YHLDLLVVGV MLGVCSVMGL PWFVAATVLS bmy_08829T0/1-1141 QQITAVIINR KEHKLKKGAG YHLDLLVVGV MLGVCSVMGL PWFVAATVLS BACU014668/1-1379 ---------- ------KGAG YHLDLLVVGV MLGVCSVMGL PWFVAATVLS ENSTTRP00000010232/1-1198 QQITAVIINR KEHKLKKGAG YHLDLLVVGV MLGVCSVMGL PWFVAATVLS 1051 1100 ENSP00000295736/1-1214 ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK ENSBTAP00000053881/1-1218 ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK bmy_08829T0/1-1141 ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK BACU014668/1-1379 ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK ENSTTRP00000010232/1-1198 ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK 1101 1150 ENSP00000295736/1-1214 F-IPMPVLYG VFLYMGVSS- ---------- ---------- --LKGIQLFD ENSBTAP00000053881/1-1218 F-IPMPVLYG VFLYMGVSS- ---------- ---------- --LKGIQFFD bmy_08829T0/1-1141 VKISLLQCFK IFLYHKIYTW LCETLKYLKL VSFPRIYLEN NILEWWLFFD BACU014668/1-1379 ---------- ---------- ---------- ---------- -------FFD ENSTTRP00000010232/1-1198 F-IPMPVLYG VFLYMGVSS- ---------- ---------- --LKGIQFFD 1151 1200 ENSP00000295736/1-1214 RIKLFGMPAK HQPDLIYLRY VPLWKVHIFT VIQLTCLVLL WVIKVSAAAV ENSBTAP00000053881/1-1218 RIKLFGMPAK HQPDLIYLRY VPLWKVHVFT VVQLTCLVLL WVIKASAAAV bmy_08829T0/1-1141 RIKLFGMPAK HQPDLIYLRY VPLWKVHVFT VVQLTCLILL WVIKASAAAV BACU014668/1-1379 RIKLFGMPAK HQPDLIYLRY VPLWKVHVFT VVQLTCLILL WVIKASAAAV ENSTTRP00000010232/1-1198 RIKLFGMPAK HQPDLIYLRY VPLWKVHVFT VVQLTCLILL WVIKASAAAV 1201 1250 ENSP00000295736/1-1214 VFPMMVLALV FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE ENSBTAP00000053881/1-1218 VFPMMVLALV FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE bmy_08829T0/1-1141 VFPMMVLALV FVRKLMDLCF TKRELSWLDD LMPET----- ---------- BACU014668/1-1379 VFPMMVLALV FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE ENSTTRP00000010232/1-1198 VFPMMVLALV FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE 1251 1300 ENSP00000295736/1-1214 AERMLQDDDD TVHLPFEGGS LLQIPVKALK Y--------- ---------- ENSBTAP00000053881/1-1218 AERMLQAGED TVHLPFEGGS LLQIPVKALK Y--------- ---------- bmy_08829T0/1-1141 -ERMLQAGED TVHLPFEGGS LLQIPVKALK YSVDPSVVNI SDEMAK---- BACU014668/1-1379 AERMLQAGED TVHLPFEGGS LLQIPVKALK YRLPACYSTT FDACCKLYLT ENSTTRP00000010232/1-1198 AERMLQAGED TVHLPFEGGS LLQIPVKALK Y--------- ---------- 1301 1350 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 ----TAQWKA LSMNTENAKV TRSNT----- ---------- ---------- BACU014668/1-1379 KNRGKEGLPA INFDSARSSM TKSEPARAGG HRARGQWHKS TEAIELENFR ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 1351 1400 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ---------- BACU014668/1-1379 EWVDRAPSLH FLRVLICLRL LMRDPCYQYM EIVAHGYLGS GEAQHSVDKL ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 1401 1450 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ---------- BACU014668/1-1379 VNMTYIFQKL AAVKDQREWV TTSGAHKTLV NLLSARDTNV LLGALLALAS ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 1451 1500 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ---------- BACU014668/1-1379 LAERLTAELL RLLCAERQVR EKVKLYEGVP ILLSLLHSDH LQLLWSVVWI ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 1501 1550 ENSP00000295736/1-1214 ------SPDK PVSVKI---- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ------SPEK PVSVKI---- ---------- ---------- ---------- bmy_08829T0/1-1141 ------SPEK PVSVKI---- ---------- ---------- ---------- BACU014668/1-1379 LVQVCEDPET SVEIRIWGGI KQILHILRGD RNFVSDHSSI GSLSSANAAG ENSTTRP00000010232/1-1198 ------SPEK PVSVKI---- ---------- ---------- ---------- 1551 1600 ENSP00000295736/1-1214 ---------- ---------S F--------- ---------- ------EDE- ENSBTAP00000053881/1-1218 ---------- ---------N F--------- ---------- ------EDE- bmy_08829T0/1-1141 ---------- ---------S F--------- ---------- ------EDE- BACU014668/1-1379 RIQQLHLSED LSPREIQENT FSLQAACCAA LTELVLDDTN AHQVVQEDEL ENSTTRP00000010232/1-1198 ---------- ---------S F--------- ---------- ------EDE- 1601 1650 ENSP00000295736/1-1214 ---------- -----PRK-- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- -----PRK-- ---------- ---------- ---------- bmy_08829T0/1-1141 ---------- -----PTK-- ---------- ---------- ---------- BACU014668/1-1379 KQIAENIESI NQNKAPSKYI GNYAVLDHLG SGAFGCVYKV RKRSGQNLLA ENSTTRP00000010232/1-1198 ---------- -----PTK-- ---------- ---------- ---------- 1651 1700 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- KYVDAETSL- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- KYLDAETSL- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- KYMDAETSL- BACU014668/1-1379 MKEVNLHNPA FGKDKKDRDS SVRNIVSELT IIKEQLCLAL RYLHKEKRII ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- KYMDAETSL- 1701 1750 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- ---------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- ---------- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- ---------- BACU014668/1-1379 HRDLTPNNIM LGDKDRVTVS KYKNLKIFNL RVSEYYSRTE LFDRTFCTEW ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- ---------- 1751 1800 ENSP00000295736/1-1214 ---------- ---------- ---------- ---------- -------- ENSBTAP00000053881/1-1218 ---------- ---------- ---------- ---------- -------- bmy_08829T0/1-1141 ---------- ---------- ---------- ---------- -------- BACU014668/1-1379 RVLCLHCLVW QPLATCGYQA LETCLIACVP EDMNFKFYQI LVNLNIPM ENSTTRP00000010232/1-1198 ---------- ---------- ---------- ---------- --------